In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 15
... HEPES buffer to make its concentra- tion 10 mM in the fully constituted medium , and 12 parts fetal calf serum . The L - glutamine , which is supplied frozen and in 100 × concentration ( 200 mM ) , is thawed , and 1 ml is added to 100 ...
... HEPES buffer to make its concentra- tion 10 mM in the fully constituted medium , and 12 parts fetal calf serum . The L - glutamine , which is supplied frozen and in 100 × concentration ( 200 mM ) , is thawed , and 1 ml is added to 100 ...
Page 256
... ( HEPES ) : Prepare 500 ml of a 1 M solution of HEPES . Filter through a 0.2 - μm filter to sterilize and store at 4 ° C . Sodium hydroxide ( NaOH ) : Prepare a 1 N solution and filter through a 0.2 - μm filter to sterilize . Store at 4 ...
... ( HEPES ) : Prepare 500 ml of a 1 M solution of HEPES . Filter through a 0.2 - μm filter to sterilize and store at 4 ° C . Sodium hydroxide ( NaOH ) : Prepare a 1 N solution and filter through a 0.2 - μm filter to sterilize . Store at 4 ...
Page 312
... HEPES , and 0.4 ml gentamicin sulfate . Filter the solution through a 0.2 - μm bottle - top filter ; the pH should be 7.4 ± 0.1 . Solution II : HEPES , NaOH , and gentamicin sulfate Collagenase D ( Boehringer - Mannheim ) Williams ...
... HEPES , and 0.4 ml gentamicin sulfate . Filter the solution through a 0.2 - μm bottle - top filter ; the pH should be 7.4 ± 0.1 . Solution II : HEPES , NaOH , and gentamicin sulfate Collagenase D ( Boehringer - Mannheim ) Williams ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml