In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
From inside the book
Results 1-3 of 51
Page 117
... ( Sigma , St. Louis , MO , M - 0518 ) Dulbecco's modified Eagle's medium ( DME ) and Ham's nutrient mixture F12 ( Sigma D - 8900 ) Trypsin , type IX from porcine pancreas ( Sigma T - 0134 ) Deoxyribonuclease I from bovine pancreas , Type IV ( ...
... ( Sigma , St. Louis , MO , M - 0518 ) Dulbecco's modified Eagle's medium ( DME ) and Ham's nutrient mixture F12 ( Sigma D - 8900 ) Trypsin , type IX from porcine pancreas ( Sigma T - 0134 ) Deoxyribonuclease I from bovine pancreas , Type IV ( ...
Page 245
... Sigma ( St. Louis , MO ) in the highest purity available . Chemicals needed for the dissociation of liver cells include Hanks ' calcium - free BSS ( Table I ) , collagenase type IV ( Worthington , Freehold , NJ , Cat . No. 4189 ) , and ...
... Sigma ( St. Louis , MO ) in the highest purity available . Chemicals needed for the dissociation of liver cells include Hanks ' calcium - free BSS ( Table I ) , collagenase type IV ( Worthington , Freehold , NJ , Cat . No. 4189 ) , and ...
Page 282
... ( Sigma , A - 0884 ) 112 mg 128 mg 240 mg Dissolve into 31 ml sterile distilled water Dispense amino acid stock ... ( Sigma ) 7.5 % NaHCO3 ( GIBCO ) 1 M HEPES ( GIBCO ) Hormone mixture stock solutions Oleic acid ( Sigma , O - 7501 ) Linoleic ...
... ( Sigma , A - 0884 ) 112 mg 128 mg 240 mg Dissolve into 31 ml sterile distilled water Dispense amino acid stock ... ( Sigma ) 7.5 % NaHCO3 ( GIBCO ) 1 M HEPES ( GIBCO ) Hormone mixture stock solutions Oleic acid ( Sigma , O - 7501 ) Linoleic ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml