In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 404
... activity was measured at 410 nm as formation of p - nitrophenol with p - nitrophenylphosphate as substrate . Hexokinase activity was measured at 340 nm by an NADPH - linked assay . Cellular oxygen consumption was measured with a Clark ...
... activity was measured at 410 nm as formation of p - nitrophenol with p - nitrophenylphosphate as substrate . Hexokinase activity was measured at 340 nm by an NADPH - linked assay . Cellular oxygen consumption was measured with a Clark ...
Page 496
... activity , or a loss of adherent cells producing the activity , would be reflected by a decrease in myeloid colonies in the agar overlayer . We noted , however , that occasionally the adherent layer in control cultures would overgrow ...
... activity , or a loss of adherent cells producing the activity , would be reflected by a decrease in myeloid colonies in the agar overlayer . We noted , however , that occasionally the adherent layer in control cultures would overgrow ...
Page 498
... activity and simultaneously establish a baseline of this activity which can then be used for comparison after drug exposure . We have recently adapted the procedure to 6 - well culture plates and have noted an improvement in the degree ...
... activity and simultaneously establish a baseline of this activity which can then be used for comparison after drug exposure . We have recently adapted the procedure to 6 - well culture plates and have noted an improvement in the degree ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml