In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
From inside the book
Results 1-3 of 77
Page 125
... Buffer A : The following stock reagents are required : 0.9 % NaCl ; 0.15 M KCI ; 0.1 M sodium phosphate buffer , pH 7.4 ; 0.2 M HEPES brought to pH 7.4 with 8 M NaOH ; glucose ; and gentamicin ( Whittaker M. A. Bioproducts ...
... Buffer A : The following stock reagents are required : 0.9 % NaCl ; 0.15 M KCI ; 0.1 M sodium phosphate buffer , pH 7.4 ; 0.2 M HEPES brought to pH 7.4 with 8 M NaOH ; glucose ; and gentamicin ( Whittaker M. A. Bioproducts ...
Page 235
... buffer ( 0.05 % w / v collagenase ) : 10 ml collagenase buffer con- centrate , water to 50 ml ; pH 7.6 at 37 ° C . Store at 0 ° C in a 50 - ml measur- ing cylinder covered with Parafilm . The buffer should be warmed to and maintained at ...
... buffer ( 0.05 % w / v collagenase ) : 10 ml collagenase buffer con- centrate , water to 50 ml ; pH 7.6 at 37 ° C . Store at 0 ° C in a 50 - ml measur- ing cylinder covered with Parafilm . The buffer should be warmed to and maintained at ...
Page 399
... buffer Henseleit buffer Ca2 + -Free , low- Mg2 + K - H buffer Component Α1 A2 B1 B2 C1 C2 1. NaCl ( mM ) 137 137 118 118 118 118 2. KCl ( mM ) 5.4 5.4 4.8 4.8 4.8 4.8 E 3. MgSO4 7H2O ( mM ) 0.8 0.8 1.2 1.2 0.12 0.12 4. KH2PO4 ( mM ) 0.4 ...
... buffer Henseleit buffer Ca2 + -Free , low- Mg2 + K - H buffer Component Α1 A2 B1 B2 C1 C2 1. NaCl ( mM ) 137 137 118 118 118 118 2. KCl ( mM ) 5.4 5.4 4.8 4.8 4.8 4.8 E 3. MgSO4 7H2O ( mM ) 0.8 0.8 1.2 1.2 0.12 0.12 4. KH2PO4 ( mM ) 0.4 ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml