In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 46
... Cell Culture = Kamala Rose , Mark P. Goldberg , and Dennis W. Choi INTRODUCTION Primary cell culture derived from nervous system tissue appears to be a useful system for the study of neurotoxic agents . Neuronal cultures permit the ...
... Cell Culture = Kamala Rose , Mark P. Goldberg , and Dennis W. Choi INTRODUCTION Primary cell culture derived from nervous system tissue appears to be a useful system for the study of neurotoxic agents . Neuronal cultures permit the ...
Page 541
... culture , 527-528 Epidermis cell types in , 525 differentiated keratinocytes in , 529 layers of , 525 metabolic capabilities of , 526 Epithelial cells . See Alveolar epithelial cells ; Clara cells ; Corneal epithelial cell culture ...
... culture , 527-528 Epidermis cell types in , 525 differentiated keratinocytes in , 529 layers of , 525 metabolic capabilities of , 526 Epithelial cells . See Alveolar epithelial cells ; Clara cells ; Corneal epithelial cell culture ...
Page 552
... cell cultures ( cont . ) plating for , 51 neurotoxin exposure in , assessment of injury from , 53-57 preparation for , 58 procedures for , 48-53 prolonged neurotoxin exposure in , 53 solutions for , 46-48 surfaces for , 58 ...
... cell cultures ( cont . ) plating for , 51 neurotoxin exposure in , assessment of injury from , 53-57 preparation for , 58 procedures for , 48-53 prolonged neurotoxin exposure in , 53 solutions for , 46-48 surfaces for , 58 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml