In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 10
... water from the laboratory . Distilled water used in the collagen preparation and coating procedures is also ... Deionized water is used for most glassware cleaning steps . For making up solu- tions that will be applied to cultures ...
... water from the laboratory . Distilled water used in the collagen preparation and coating procedures is also ... Deionized water is used for most glassware cleaning steps . For making up solu- tions that will be applied to cultures ...
Page 12
... deionized wa- ter , the glassware is transferred to stainless steel utility tanks containing 8 liters of deionized water to which has been added 160 ml of the stock sodium metasilicate solution . The mixture is brought to a boil , and ...
... deionized wa- ter , the glassware is transferred to stainless steel utility tanks containing 8 liters of deionized water to which has been added 160 ml of the stock sodium metasilicate solution . The mixture is brought to a boil , and ...
Page 13
... deionized water and 50 ml of stock Ivory soap solution , and boiled for 10 min . Subsequently the racks with the slides are transferred to three changes of boil- ing deionized water without soap and boiled for 10 min in each change ...
... deionized water and 50 ml of stock Ivory soap solution , and boiled for 10 min . Subsequently the racks with the slides are transferred to three changes of boil- ing deionized water without soap and boiled for 10 min in each change ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml