In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 177
... developed force . Beyond the plateau phase only two increments that result in less developed force are usu- ally necessary to characterize the descending portion of the length- tension - relationship adequately . Resulting contractions ...
... developed force . Beyond the plateau phase only two increments that result in less developed force are usu- ally necessary to characterize the descending portion of the length- tension - relationship adequately . Resulting contractions ...
Page 180
... developed that becomes routine while remaining carefully thorough and complete . In our laboratories both simple and opened rings are preferred over spiral strips for these kinds of analyses for several reasons . Early studies clearly ...
... developed that becomes routine while remaining carefully thorough and complete . In our laboratories both simple and opened rings are preferred over spiral strips for these kinds of analyses for several reasons . Early studies clearly ...
Page 366
... developed for primary kid- ney epithelial cell cultures , as well as for established kidney epithelial cell lines ( 1-3 ) . The hormonally defined media developed for primary kidney epithelial cells have been particularly effective in ...
... developed for primary kid- ney epithelial cell cultures , as well as for established kidney epithelial cell lines ( 1-3 ) . The hormonally defined media developed for primary kidney epithelial cells have been particularly effective in ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml