In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 23
... explants , as illustrated in Fig . 3. A spinal cord explant with DRG at- tached by dorsal roots is shown in Fig . 3A . The silver - stained culture in Fig . 3B was photographed with dark - field optics to demonstrate the rich plexus of ...
... explants , as illustrated in Fig . 3. A spinal cord explant with DRG at- tached by dorsal roots is shown in Fig . 3A . The silver - stained culture in Fig . 3B was photographed with dark - field optics to demonstrate the rich plexus of ...
Page 206
... Explants , 1 mm thick , are fixed in 2 % phosphate- buffered glutaraldehyde for 1-2 hr at 4 ° C . They are ... Explants For each medium , at a chosen time point , the preservation of tissue can vary both between and within specimens ...
... Explants , 1 mm thick , are fixed in 2 % phosphate- buffered glutaraldehyde for 1-2 hr at 4 ° C . They are ... Explants For each medium , at a chosen time point , the preservation of tissue can vary both between and within specimens ...
Page 209
... explants , however , focal areas of crypt loss are evident in the center of the tissue . A similar picture is seen on days 5 and 7 ( Fig . 5 ) where the viability of explants remains between 60 and 70 % . In our experience if tissue is ...
... explants , however , focal areas of crypt loss are evident in the center of the tissue . A similar picture is seen on days 5 and 7 ( Fig . 5 ) where the viability of explants remains between 60 and 70 % . In our experience if tissue is ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml