In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 83
... fetal calf serum , and 1 % penicillin - streptomycin as described for growth medium Krebs - Ringer solution ( in mM ) : NaCl , 125 ; KCl , 5 ; HEPES - NaOH , 25 ; glucose , 6 ; NaHCO3 , 5 ; MgSO4 , 1.2 ; KH2PO4 , 1.2 ; and CaCl2 , 1.2 ...
... fetal calf serum , and 1 % penicillin - streptomycin as described for growth medium Krebs - Ringer solution ( in mM ) : NaCl , 125 ; KCl , 5 ; HEPES - NaOH , 25 ; glucose , 6 ; NaHCO3 , 5 ; MgSO4 , 1.2 ; KH2PO4 , 1.2 ; and CaCl2 , 1.2 ...
Page 244
... fetal hepatocytes have certain limitations . For example , fetal liver cells grow and divide rapidly , which is not characteristic of young or adult liver cells ; in addition , several enzymes found in older liver cells , such as NADPH ...
... fetal hepatocytes have certain limitations . For example , fetal liver cells grow and divide rapidly , which is not characteristic of young or adult liver cells ; in addition , several enzymes found in older liver cells , such as NADPH ...
Page 273
... fetal calf serum under a humidified atmosphere composed of 95 % air / 5 % CO2 ( 2.5 × 10 ° cells / 4 ml / 60 - mm dish ; 0.8 × 106 cells / 2 ml / 35 - mm dish ; 0.15 × 106 cells / 0.5 ml / well in 24 - well dish ; 3 × 10 cells / 0.1 ml ...
... fetal calf serum under a humidified atmosphere composed of 95 % air / 5 % CO2 ( 2.5 × 10 ° cells / 4 ml / 60 - mm dish ; 0.8 × 106 cells / 2 ml / 35 - mm dish ; 0.15 × 106 cells / 0.5 ml / well in 24 - well dish ; 3 × 10 cells / 0.1 ml ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml