In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 47
... Filter and store at 4 ° C for up to 12 months . Dilute 1:10 with sterile water before use . Trypsin ( 0.25 % ) 0.25 g trypsin ( Sigma , St. Louis , MO , T - 6763 ) 100 ml 1 × dissecting medium Filter , freeze in aliquots at -70 ° C for ...
... Filter and store at 4 ° C for up to 12 months . Dilute 1:10 with sterile water before use . Trypsin ( 0.25 % ) 0.25 g trypsin ( Sigma , St. Louis , MO , T - 6763 ) 100 ml 1 × dissecting medium Filter , freeze in aliquots at -70 ° C for ...
Page 106
... filter . The com- partment within the upper part of the cylinder contains the apical membrane fluid ; the compartment below the filter in continuity with the culture plate itself contains the basal fluid . The movement of fluid can only ...
... filter . The com- partment within the upper part of the cylinder contains the apical membrane fluid ; the compartment below the filter in continuity with the culture plate itself contains the basal fluid . The movement of fluid can only ...
Page 256
... Filter through a 0.2 - μm filter to sterilize and store at 4 ° C . Sodium hydroxide ( NaOH ) : Prepare a 1 N solution and filter through a 0.2 - μm filter to sterilize . Store at 4 ° C . Ethylene glycol bis ( B - aminoethyl ether ) -N ...
... Filter through a 0.2 - μm filter to sterilize and store at 4 ° C . Sodium hydroxide ( NaOH ) : Prepare a 1 N solution and filter through a 0.2 - μm filter to sterilize . Store at 4 ° C . Ethylene glycol bis ( B - aminoethyl ether ) -N ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml