In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 256
... final concentration of 10 mM and 1 N NaOH to adjust the pH to 7.35 ( ~ 0.8 ml to 200 ml of the EGTA solution ) . Add gentamicin to a final concentration of 50 μg / ml . This solution should be freshly prepared . Collagenase ...
... final concentration of 10 mM and 1 N NaOH to adjust the pH to 7.35 ( ~ 0.8 ml to 200 ml of the EGTA solution ) . Add gentamicin to a final concentration of 50 μg / ml . This solution should be freshly prepared . Collagenase ...
Page 399
... final suspension of cortical cells ; B2 , buffer for final suspension of proximal tubular and distal tubular cells ; C1 , Percoll separation buffer ; C2 , Percoll wash buffer . Abbreviations : HEPES , 4- ( 2 - hydroxyethyl ) -1 ...
... final suspension of cortical cells ; B2 , buffer for final suspension of proximal tubular and distal tubular cells ; C1 , Percoll separation buffer ; C2 , Percoll wash buffer . Abbreviations : HEPES , 4- ( 2 - hydroxyethyl ) -1 ...
Page 494
... final concentration ) 0.1 ml sodium pyruvate solution ( 10 μM final concentration ) 0.1 ml 2 - mercaptoethanol solution ( 50 μM final concentration ) 7.5 ml FBS 7.5 ml HS 84.3 ml RPMI 1640 medium with glutamine 100.0 ml Procedure ...
... final concentration ) 0.1 ml sodium pyruvate solution ( 10 μM final concentration ) 0.1 ml 2 - mercaptoethanol solution ( 50 μM final concentration ) 7.5 ml FBS 7.5 ml HS 84.3 ml RPMI 1640 medium with glutamine 100.0 ml Procedure ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml