In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 33
... flasks are then capped with rubber stoppers ( Size 0 ) and placed in a rotatory incubator at 80 rpm ( New Brunswick Sci- entific , New Brunswick , NJ ; Model No. G - 25 ) at 37 ° C . After the first 24 hr , the culture flask is tilted ...
... flasks are then capped with rubber stoppers ( Size 0 ) and placed in a rotatory incubator at 80 rpm ( New Brunswick Sci- entific , New Brunswick , NJ ; Model No. G - 25 ) at 37 ° C . After the first 24 hr , the culture flask is tilted ...
Page 38
... flask ( Poisson parameter Ac Ve Ye ) and one treated flask ( Poisson parameter λt V1y1 ) . The test is based on the null hy- pothesis that these two parameters are the same , i.e. there is no difference be- tween expected cell numbers ...
... flask ( Poisson parameter Ac Ve Ye ) and one treated flask ( Poisson parameter λt V1y1 ) . The test is based on the null hy- pothesis that these two parameters are the same , i.e. there is no difference be- tween expected cell numbers ...
Page 43
... flask is critical for establishing the proper rate of rotation . The rate must be increased with increasing volume ... flask and ad- here to it . The adequacy of the rotation rate can be judged from the following : if it is too fast ...
... flask is critical for establishing the proper rate of rotation . The rate must be increased with increasing volume ... flask and ad- here to it . The adequacy of the rotation rate can be judged from the following : if it is too fast ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml