In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 409
... functional groups on protein or nucleic acids . As moni- tored by lactate dehydrogenase leakage ( Fig . 3 ) , MVK was markedly more cyto- toxic in DT cells than in PT cells , consistent with the pattern observed with per- oxides and ...
... functional groups on protein or nucleic acids . As moni- tored by lactate dehydrogenase leakage ( Fig . 3 ) , MVK was markedly more cyto- toxic in DT cells than in PT cells , consistent with the pattern observed with per- oxides and ...
Page 553
... functional variables , 24 glassware for , 8-9 materials for processing , 9 metasilicate procedure for , 12 processing procedures for , 11-13 sterilization of , 10 instruments for , 9–10 sterilization of , 10 light microscopic analysis ...
... functional variables , 24 glassware for , 8-9 materials for processing , 9 metasilicate procedure for , 12 processing procedures for , 11-13 sterilization of , 10 instruments for , 9–10 sterilization of , 10 light microscopic analysis ...
Page 562
... functional integrity after , 334-337 succinate oxidation after , 335–336 comparison of techniques for , 348 with magnetic iron oxide , 331–333 applications of , 337-338 compared with collagenase method , 333 structural and functional ...
... functional integrity after , 334-337 succinate oxidation after , 335–336 comparison of techniques for , 348 with magnetic iron oxide , 331–333 applications of , 337-338 compared with collagenase method , 333 structural and functional ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml