In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 353
... kidneys . Only part of the kidney may be obtained , and in this case a cannula should be inserted into a vessel or space on the cut surface . Only a portion of the tissue will be perfused , but sufficient quantities of proximal tubules ...
... kidneys . Only part of the kidney may be obtained , and in this case a cannula should be inserted into a vessel or space on the cut surface . Only a portion of the tissue will be perfused , but sufficient quantities of proximal tubules ...
Page 366
... kidney cells from the species of interest can be examined , so that in vitro studies need not be restricted to a cell lineage that has fortuitously been established in culture . However , until recently , the use of primary kidney cell ...
... kidney cells from the species of interest can be examined , so that in vitro studies need not be restricted to a cell lineage that has fortuitously been established in culture . However , until recently , the use of primary kidney cell ...
Page 369
... kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated kidneys are conve- nient for sterile transport to the tissue culture laboratory without spillage of medium . Each kidney is then placed in a sterile 100 ...
... kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated kidneys are conve- nient for sterile transport to the tissue culture laboratory without spillage of medium . Each kidney is then placed in a sterile 100 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml