In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 89
... measured before the addition of test compounds , and fluorescence is measured at dual excitation wavelengths of 340 and 380 nm with emission wavelength of 510 nm . Cytosolic free calcium levels are determined by the following formula ...
... measured before the addition of test compounds , and fluorescence is measured at dual excitation wavelengths of 340 and 380 nm with emission wavelength of 510 nm . Cytosolic free calcium levels are determined by the following formula ...
Page 401
... measured ( Fig . 1B ) . Alkaline phosphatase ( EC 3.1.3.1 ) and y - glutamyltransferase ( EC 2.3.2.2 ) activities are measured as markers for the PT cells , and they are recovered predominantly in fractions 1 to 4. In contrast , hex ...
... measured ( Fig . 1B ) . Alkaline phosphatase ( EC 3.1.3.1 ) and y - glutamyltransferase ( EC 2.3.2.2 ) activities are measured as markers for the PT cells , and they are recovered predominantly in fractions 1 to 4. In contrast , hex ...
Page 404
... measured at 340 nm with pyruvate and NADH in the absence and presence of Triton X - 100 . y - Glutamyltransferase activity was measured at 410 nm as formation of p - nitroanilide with y glutamyl - p - nitroanilide and glycylglycine as ...
... measured at 340 nm with pyruvate and NADH in the absence and presence of Triton X - 100 . y - Glutamyltransferase activity was measured at 410 nm as formation of p - nitroanilide with y glutamyl - p - nitroanilide and glycylglycine as ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml