In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 231
... Perfusion Per O. Seglen INTRODUCTION The availability of methods for isolation of large quantities of intact cells has made isolated hepatocytes and hepatocyte cultures a favorite experimental sys- tem for pharmacological ...
... Perfusion Per O. Seglen INTRODUCTION The availability of methods for isolation of large quantities of intact cells has made isolated hepatocytes and hepatocyte cultures a favorite experimental sys- tem for pharmacological ...
Page 265
... perfusion method used in the isolation of human hepatocytes is dependent on the size of the specimen received . When the whole liver is obtained , or at least a major portion of it , the big biopsy perfusion method is conducted . For ...
... perfusion method used in the isolation of human hepatocytes is dependent on the size of the specimen received . When the whole liver is obtained , or at least a major portion of it , the big biopsy perfusion method is conducted . For ...
Page 267
... perfusion tubing . The rubber stopper of the perfusion tower fits into a 600 - ml beaker . The tower can easily be sterilized in an autoclave . 2. Prior to use , the tubing is sterilized by circulating 70 % ethanol through it for a ...
... perfusion tubing . The rubber stopper of the perfusion tower fits into a 600 - ml beaker . The tower can easily be sterilized in an autoclave . 2. Prior to use , the tubing is sterilized by circulating 70 % ethanol through it for a ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml