In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 9
... pipettes ( Bellco , Vineland , NJ ) are the mainstay of most of the culture feeding and fluid transferring operations . One other variety of pipette that is used is a 10 - ml graduated serological pipette with a large bore tip ( Kimax ...
... pipettes ( Bellco , Vineland , NJ ) are the mainstay of most of the culture feeding and fluid transferring operations . One other variety of pipette that is used is a 10 - ml graduated serological pipette with a large bore tip ( Kimax ...
Page 51
... pipettes and use a flame to reduce the bore size of one pipette to one - third of normal . Pour out an appropriate amount of plating medium into a flask and set aside ( 2.75 hemispheres / plate or 2.75 hemispheres / 9.6 ml of PM gives ...
... pipettes and use a flame to reduce the bore size of one pipette to one - third of normal . Pour out an appropriate amount of plating medium into a flask and set aside ( 2.75 hemispheres / plate or 2.75 hemispheres / 9.6 ml of PM gives ...
Page 298
... pipetting with a ster- ile 10 - ml plastic pipette . 7. Layer the 25 ml of cell suspension evenly across the four gradient tubes using a sterile transfer pipette . 8. Centrifuge the gradients in an SW - 40 rotor at 20,000 rpm for 25 min ...
... pipetting with a ster- ile 10 - ml plastic pipette . 7. Layer the 25 ml of cell suspension evenly across the four gradient tubes using a sterile transfer pipette . 8. Centrifuge the gradients in an SW - 40 rotor at 20,000 rpm for 25 min ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml