In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 19
... placed on the upper tier of the work shelf , along with a small beaker containing autoclaved petroleum jelly and a sterile glass rod . Cultures are fed in groups of four . Two sterile square coverslips are placed in each of the two 20 x ...
... placed on the upper tier of the work shelf , along with a small beaker containing autoclaved petroleum jelly and a sterile glass rod . Cultures are fed in groups of four . Two sterile square coverslips are placed in each of the two 20 x ...
Page 151
... placed into centrifuge tubes . The tubes are centrifuged in a clinical table top centrifuge at a speed of approximately 3000 rpm for 3 to 4 min . The resultant supernatant is removed and discarded , and 5 ml of MEM with antibiotics is ...
... placed into centrifuge tubes . The tubes are centrifuged in a clinical table top centrifuge at a speed of approximately 3000 rpm for 3 to 4 min . The resultant supernatant is removed and discarded , and 5 ml of MEM with antibiotics is ...
Page 369
... placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing the kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated ...
... placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing the kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml