In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
From inside the book
Results 1-3 of 47
Page 130
... present as determined by using a light polarization assay ( 32 ) . Anti- bodies to a cytochrome P - 450 isozyme characteristic of mouse Clara cells in situ , but not type II cells , reacted with most of the isolated cells ( 10 ) ...
... present as determined by using a light polarization assay ( 32 ) . Anti- bodies to a cytochrome P - 450 isozyme characteristic of mouse Clara cells in situ , but not type II cells , reacted with most of the isolated cells ( 10 ) ...
Page 200
... present method provides a preparation of cells that are use- ful for diverse studies of toxicity and mechanisms to protect against injury in the small intestine . This same method may also be used for preparation of cells from other ...
... present method provides a preparation of cells that are use- ful for diverse studies of toxicity and mechanisms to protect against injury in the small intestine . This same method may also be used for preparation of cells from other ...
Page 369
... present , greater care should now be taken to maintain sterility . Using sterile , curved - nosed scissors , the kidney is sliced longitudinally into 3 - mm - thick slices , starting from the outer tissue ( the cortex ) and gradually ...
... present , greater care should now be taken to maintain sterility . Using sterile , curved - nosed scissors , the kidney is sliced longitudinally into 3 - mm - thick slices , starting from the outer tissue ( the cortex ) and gradually ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml