In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 108
... removed indicates a good separation . The neural retina is cut at the optic disc and removed . In fact sheets of RPE are teased from Bruch's membrane with a pair of jeweler's forceps . It is important not to puncture Bruch's membrane in ...
... removed indicates a good separation . The neural retina is cut at the optic disc and removed . In fact sheets of RPE are teased from Bruch's membrane with a pair of jeweler's forceps . It is important not to puncture Bruch's membrane in ...
Page 161
... removed by laying the scissors flat between the spinal cord and the aorta and cutting caudally as the free end of the vessel is gently lifted away from the scissors . The vessel is immediately placed in BSS at 4 ° C . This salt solution ...
... removed by laying the scissors flat between the spinal cord and the aorta and cutting caudally as the free end of the vessel is gently lifted away from the scissors . The vessel is immediately placed in BSS at 4 ° C . This salt solution ...
Page 424
... removed simulta- neously by grasping Reichert's membrane at the uppermost pole of the VYS ( presomite stages ) or at one side of the VYS ( early somite and later stages ) and pulling the membrane apart . Removing Reichert's membrane is ...
... removed simulta- neously by grasping Reichert's membrane at the uppermost pole of the VYS ( presomite stages ) or at one side of the VYS ( early somite and later stages ) and pulling the membrane apart . Removing Reichert's membrane is ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml