In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 62
... solution , 25 ml of nutrient broth solution , and 11.1 ml of 90 mM CaC12 · 2H2O stock solution in demineralized , distilled water . The pH is adjusted to 7.35 with 1 N NaOH . The solution ( 1050 ml final volume ) is sterilized by ...
... solution , 25 ml of nutrient broth solution , and 11.1 ml of 90 mM CaC12 · 2H2O stock solution in demineralized , distilled water . The pH is adjusted to 7.35 with 1 N NaOH . The solution ( 1050 ml final volume ) is sterilized by ...
Page 118
Charles A. Tyson, John M. Frazier. Solutions Saline solution ( pH 7.4 ) : 0.85 % NaCl ( 8.5 g / liter ) 3 mM K2HPO4 ( 0.523 g / liter ) 5 mM Tris ( 0.606 g / liter ) 3 mM EDTA FBSS ( pH 7.4 ) , sterile filter : ( 10 ml / liter of 300 mM ...
Charles A. Tyson, John M. Frazier. Solutions Saline solution ( pH 7.4 ) : 0.85 % NaCl ( 8.5 g / liter ) 3 mM K2HPO4 ( 0.523 g / liter ) 5 mM Tris ( 0.606 g / liter ) 3 mM EDTA FBSS ( pH 7.4 ) , sterile filter : ( 10 ml / liter of 300 mM ...
Page 314
... solution followed by perfusion with a collagenase solution . Step 1 : Preliminary Setup for Hepatocyte Isolation . Both solution I and solu- tion II should be placed in a water bath set to 42 ° C prior to the perfusion , and solution I ...
... solution followed by perfusion with a collagenase solution . Step 1 : Preliminary Setup for Hepatocyte Isolation . Both solution I and solu- tion II should be placed in a water bath set to 42 ° C prior to the perfusion , and solution I ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml