In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
From inside the book
Results 1-3 of 79
Page 189
... specific " 1Cr release over 60 min . Each bar represents the mean ± SEM . Parentheses indicate the number of separate cultures . Symbols indicate statistically significant differences ( † p < 0.01 compared to control ; * p < 0.05 , ** p ...
... specific " 1Cr release over 60 min . Each bar represents the mean ± SEM . Parentheses indicate the number of separate cultures . Symbols indicate statistically significant differences ( † p < 0.01 compared to control ; * p < 0.05 , ** p ...
Page 260
... specific functions . The disad- vantage is that these important characteristics generally tend to decline with time in culture . Initial studies showed that there was a species - specific loss of cytochrome P - 450 with increased ...
... specific functions . The disad- vantage is that these important characteristics generally tend to decline with time in culture . Initial studies showed that there was a species - specific loss of cytochrome P - 450 with increased ...
Page 407
... specific enzyme or for uptake into the cell by a specific transport system , or due to other biochemical differences that make one cell population inherently more susceptible to injury than another , can be investigated in these prepara ...
... specific enzyme or for uptake into the cell by a specific transport system , or due to other biochemical differences that make one cell population inherently more susceptible to injury than another , can be investigated in these prepara ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml