In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 14
... stored in this form at room temperature for 24-48 hr , after which they are used to set up cultures . Coverslips prepared in this way can be stored at refrigerator temperature for up to 2 weeks before use . Although commercial culture ...
... stored in this form at room temperature for 24-48 hr , after which they are used to set up cultures . Coverslips prepared in this way can be stored at refrigerator temperature for up to 2 weeks before use . Although commercial culture ...
Page 62
... stored at 4 ° C . Enzymes and Solutions for Dissociation Trypsin : Dissolve 100 mg trypsin ( type III , Sigma T - 8253 ) in 500 μl Ringer's solution ; prepare 25 - μl aliquots and store at -20 ° C . Collagenase : Dissolve 50 mg ...
... stored at 4 ° C . Enzymes and Solutions for Dissociation Trypsin : Dissolve 100 mg trypsin ( type III , Sigma T - 8253 ) in 500 μl Ringer's solution ; prepare 25 - μl aliquots and store at -20 ° C . Collagenase : Dissolve 50 mg ...
Page 349
... stored in autoclave - sterilized glass bottles or sterile plastic disposable bottles . • Perfusion buffer : A 10x stock solution is prepared and consists of : NaCl , 59.5 mg / ml ; D - glucose ( anhydrous ) , 10.0 mg / ml ; KCl , 4.0 mg ...
... stored in autoclave - sterilized glass bottles or sterile plastic disposable bottles . • Perfusion buffer : A 10x stock solution is prepared and consists of : NaCl , 59.5 mg / ml ; D - glucose ( anhydrous ) , 10.0 mg / ml ; KCl , 4.0 mg ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml