In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 169
... vessel cylinders was applied to the study of biological phenomena as early as 1906 ( 1 ) . Preparations that have evolved from the original vessel cylin- der systems include perfused vessel segments , individual strips , and simple ...
... vessel cylinders was applied to the study of biological phenomena as early as 1906 ( 1 ) . Preparations that have evolved from the original vessel cylin- der systems include perfused vessel segments , individual strips , and simple ...
Page 171
... vessel lumen or incu- bation with chemical detergents . On completion of functional measurements , the cylinders can be processed for morphologic and / or ultrastructural analysis . Because isolated vessel cylinders are only viable for ...
... vessel lumen or incu- bation with chemical detergents . On completion of functional measurements , the cylinders can be processed for morphologic and / or ultrastructural analysis . Because isolated vessel cylinders are only viable for ...
Page 172
... vessel and which has been fire - polished on both ends to minimize endothe- lial damage is first inserted through the lumen of the vessel segment . This is es- sential when using a razor blade or scalpel to prepare the strip ( see Refs ...
... vessel and which has been fire - polished on both ends to minimize endothe- lial damage is first inserted through the lumen of the vessel segment . This is es- sential when using a razor blade or scalpel to prepare the strip ( see Refs ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml