In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 53
... washed of growth medium by aspirating the volume in each well down to 125 μl and adding 750 μl of HCSS with a repeat pipettor . This step is repeated a second time ; then toxic exposure is initiated by aspirating the volume down to 125 ...
... washed of growth medium by aspirating the volume in each well down to 125 μl and adding 750 μl of HCSS with a repeat pipettor . This step is repeated a second time ; then toxic exposure is initiated by aspirating the volume down to 125 ...
Page 272
... washed via the portal vein with calcium - free HEPES buffer , pH 7.65 , then with the same buffer containing 0.05 % collagenase and 5 mM CaCl2 . The flow rate and the duration of perfusion are dependent on the size of the sample ( Table ...
... washed via the portal vein with calcium - free HEPES buffer , pH 7.65 , then with the same buffer containing 0.05 % collagenase and 5 mM CaCl2 . The flow rate and the duration of perfusion are dependent on the size of the sample ( Table ...
Page 461
... washed three times in Gey's BSS . To separate cells the pellet is resuspended in 80 ml of 50 % Percoll and centrifuged at 27,000 g at 22 ° C for 30 min , after which cells are collected from the middle zone of the gradient . The pellet ...
... washed three times in Gey's BSS . To separate cells the pellet is resuspended in 80 ml of 50 % Percoll and centrifuged at 27,000 g at 22 ° C for 30 min , after which cells are collected from the middle zone of the gradient . The pellet ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml