Molecular cloning : a laboratory manual. 3

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a good book

Contents

Chapter
8-6
Tetracycline as Regulator of Inducihie Gene Expression in Mammalian Cells 17 52
8-17
Chapter 3
8-24
INFORMATION PANEl
8-82
Differential DisplayPCR DDPCR1 8
8-96
INFORMATION PANEl
107
Chapter 1
9-1
INFORMATION PANEl
9-2
Chapter 4
11-4
Chapter 5
11-5
PROTOCOl
1-36
Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA hy 1 80
1-80
INFORMATION PANEl
1-109
INFORMATION PANEl
12-1
INFORMATION PANEl
12-94
Chapter 13
13-13

Working with Bacteriophage M13 Vectors 3 1
9-3
Radiolaheling of DNA hy Extension of Random Oligonucleotides in 9 9
9-9
Chapter 15
9-15
Synthesis of Singlestranded DNA Prohes of Heterogeneous l ength from 9 25
9-25
INFORMATION PANEl
9-76
PROTOCOl
10-1
Chapter 8
10-8
Chapter 11
10-11
INTRODUCTION
10-19
Preparation and Analysis of Eukaryotic Genomic DNA 6 1
6
In Vitro Amplification of DNA hy the Polymerase 8 1
8
PROTOCOl
11-1
INFORMATION PANEl
14-1
Chapter 14
14-14
Chapter 16
14-16
Measurement of Chloramphenicol Acetyltransferase in Extracts of Mammalian Cells 1 7 33
1
Isolation of Highmolecularweight DNA from Mammalian Cells Using Proteinase K 6 4
6
Chapter 7
17
1
1-1
Extraction Purification and Analysis of mRNA from 7 1
1-7
Eukaryotic Cells
1-17
PROTOCOl
1-25
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Page 9-93 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 23 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-90 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-94 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-89 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-89 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-107 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-90 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

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