Molecular Cloning: A Laboratory Manual, Volume 1

Front Cover
CSHL Press, 2001 - Science - 2344 pages
16 Reviews
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
 

What people are saying - Write a review

User ratings

5 stars
13
4 stars
3
3 stars
0
2 stars
0
1 star
0

LibraryThing Review

User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

LibraryThing Review

User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

Contents

Chapter
8-6
Tetracycline as Regulator of Inducihie Gene Expression in Mammalian Cells 17 52
8-17
Chapter 3
8-24
INFORMATION PANEl
8-82
Differential DisplayPCR DDPCR1 8
8-96
INFORMATION PANEl
107
Chapter 1
9-1
INFORMATION PANEl
9-2
Chapter 4
11-4
Chapter 5
11-5
PROTOCOl
1-36
Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA hy 1 80
1-80
INFORMATION PANEl
1-109
INFORMATION PANEl
12-1
INFORMATION PANEl
12-94
Chapter 13
13-13

Working with Bacteriophage M13 Vectors 3 1
9-3
Radiolaheling of DNA hy Extension of Random Oligonucleotides in 9 9
9-9
Chapter 15
9-15
Synthesis of Singlestranded DNA Prohes of Heterogeneous l ength from 9 25
9-25
INFORMATION PANEl
9-76
PROTOCOl
10-1
Chapter 8
10-8
Chapter 11
10-11
INTRODUCTION
10-19
Preparation and Analysis of Eukaryotic Genomic DNA 6 1
6
In Vitro Amplification of DNA hy the Polymerase 8 1
8
PROTOCOl
11-1
INFORMATION PANEl
14-1
Chapter 14
14-14
Chapter 16
14-16
Measurement of Chloramphenicol Acetyltransferase in Extracts of Mammalian Cells 1 7 33
1
Isolation of Highmolecularweight DNA from Mammalian Cells Using Proteinase K 6 4
6
Chapter 7
17
1
1-1
Extraction Purification and Analysis of mRNA from 7 1
1-7
Eukaryotic Cells
1-17
PROTOCOl
1-25
Copyright

Other editions - View all

Common terms and phrases

Popular passages

Page 9-93 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 23 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-90 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-94 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-89 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-89 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-107 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-90 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

References to this book

Culturing Nerve Cells
Gary Banker
Limited preview - 1998
All Book Search results »

About the author (2001)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

Bibliographic information