Molecular cloning: a laboratory manual, Volume 2 |
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Results 1-5 of 13
Page 8-26
... Step 6. Water hath preset to 75°C METHOD 1 . Pool up to eight PCRs (400 u0 containing 1 u.g of the desired amplification product. If nonspecific amplification products are present at significant levels (e.g., are detectahie hy gel ...
... Step 6. Water hath preset to 75°C METHOD 1 . Pool up to eight PCRs (400 u0 containing 1 u.g of the desired amplification product. If nonspecific amplification products are present at significant levels (e.g., are detectahie hy gel ...
Page 8-29
... step is usually suffi- cient for most subsequent manipulation steps . If necessary , trace oligonucleotide primers can be further removed by performing a second 30 - minute centrifugation step . At Step 4 above , empty the translucent ...
... step is usually suffi- cient for most subsequent manipulation steps . If necessary , trace oligonucleotide primers can be further removed by performing a second 30 - minute centrifugation step . At Step 4 above , empty the translucent ...
Page 8-33
... Step 4 . Agarose gel Please see Step 5 . Nucleic Acids and Oligonucleotides Closed circular plasmid DNA ( 50 μg / ml ) Choose a plasmid vector containing a single site for a restriction enzyme that generates blunt ends ( e.g. , Smal ...
... Step 4 . Agarose gel Please see Step 5 . Nucleic Acids and Oligonucleotides Closed circular plasmid DNA ( 50 μg / ml ) Choose a plasmid vector containing a single site for a restriction enzyme that generates blunt ends ( e.g. , Smal ...
Page 8-39
... Step 12 . Nucleic Acids and Oligonucleotides Vectors Forward primer ( 20 μM ) in H2O and Reverse primer ( 20 μM ) in ... Step 3 . Additional Reagents Step 1 of this protocol requires the reagents listed in Protocol 1 of this chapter ...
... Step 12 . Nucleic Acids and Oligonucleotides Vectors Forward primer ( 20 μM ) in H2O and Reverse primer ( 20 μM ) in ... Step 3 . Additional Reagents Step 1 of this protocol requires the reagents listed in Protocol 1 of this chapter ...
Page 8-43
... Step 5 . Nucleic Acids and Oligonucleotides Vectors Oligonucleotide primer 1 ( 10 μM ) in TE ( pH 8.0 ) and ... Step 5 of this protocol requires the reagents listed in Chapter 5 , Protocol 2 , Chapter 12 , Protocol 6 , or Chapter 6 ...
... Step 5 . Nucleic Acids and Oligonucleotides Vectors Oligonucleotide primer 1 ( 10 μM ) in TE ( pH 8.0 ) and ... Step 5 of this protocol requires the reagents listed in Chapter 5 , Protocol 2 , Chapter 12 , Protocol 6 , or Chapter 6 ...
Contents
8-6 | |
8-17 | |
8-23 | |
Analysis of Interacting Proteins with Surface Plasmon Resonance Spectroscopy 18 | 8-96 |
INFORMATION PANELS | 8-107 |
INFORMATION PANELS | 8-109 |
Chapter 9 | 8-120 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 8-128 |
Exon Trapping and Amplification 11 80 | 11-80 |
INFORMATION PANELS | 11-98 |
Chain Reaction | 11-125 |
Chapter 1 | 12-1 |
Chapter 4 | 12-4 |
Chapter 12 | 12-12 |
Chapter 16 | 12-16 |
INTRODUCTION | 12-32 |
Chapter 15 | 9-15 |
Chapter 18 | 9-18 |
INTRODUCTION | 9-25 |
PROTOCOLS | 9-33 |
INFORMATION PANELS | 9-76 |
1 | 10-1 |
Chapter 10 | 10-10 |
Volume 1 | 10-11 |
Volume 2 | 10-16 |
INFORMATION PANELS | 10-43 |
Chapter 3 | 11-3 |
Chapter 5 | 11-5 |
Chapter 11 | 11-7 |
Chapter 8 | 11-8 |
PROTOCOLS | 11-13 |
INFORMATION PANELS | 12-94 |
1 | 12-115 |
Chapter 13 | 13-13 |
INFORMATION PANELS | 13-63 |
Purification of Closed Circular DNA by Equilibrium Centrifugation in CsClEthidium 1 69 | 13-69 |
INFORMATION PANELS | 14-1 |
Chapter 2 | 14-2 |
Chapter 14 | 14-3 |
INFORMATION PANELS | 14-9 |
INFORMATION PANELS | 7 |
1 | 1-1 |
Chapter 7 | 1-7 |
Eukaryotic Cells | 1-17 |
Other editions - View all
Molecular Cloning: Selected Applications in Medicine and Biology Gregory G. Brown Limited preview - 2011 |
Common terms and phrases
Acad agarose gel aliquots amplified DNA annealing Appendix bacteriophage M13 bands base Biol BioTechniques catalyzed cDNA clones cDNA libraries cells centrifuge Chapter cleavage concentration containing cycle ddNTP denatured detection digestion DNA fragments DNA sequencing DNA templates dNTPs double-stranded DNA efficiency Escherichia coli ethanol exonuclease gel electrophoresis gene H₂O hybridization information panel Klenow fragment labeled ligation method microfuge tube microtiter plate minutes molecules mRNA mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products phagemid plaques plasmid plasmid DNA pmoles polyacrylamide gel polymerase chain reaction polymerization polynucleotide kinase preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase room temperature samples screening Sequenase sequencing gel sequencing reactions single-stranded DNA Step strand synthesis target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro wild-type µg/ml
Popular passages
Page 9-91 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 14-37 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-88 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-92 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-87 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 8-120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 8-120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-87 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-103 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-88 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.