Molecular cloning: a laboratory manual, Volume 2 |
From inside the book
Results 1-5 of 46
Page 8-1
... Amplified DNA by Ultrafiltration Introduction to Cloning PCR Products ( Protocols 4-7 ) 8.27 8.30 4 Blunt - end ... Amplified DNA 7 Genetic Engineering with PCR 8.37 8.42 8 Amplification of cDNA Generated by Reverse Transcription of mRNA ...
... Amplified DNA by Ultrafiltration Introduction to Cloning PCR Products ( Protocols 4-7 ) 8.27 8.30 4 Blunt - end ... Amplified DNA 7 Genetic Engineering with PCR 8.37 8.42 8 Amplification of cDNA Generated by Reverse Transcription of mRNA ...
Page 8-4
... , and to facilitate sub- sequent manipulation of the amplified product . Given that primers so heavily influence the success or failure of PCR protocols , it is ironic 8.4 Chapter 8 : In Vitro Amplification of DNA by PCR.
... , and to facilitate sub- sequent manipulation of the amplified product . Given that primers so heavily influence the success or failure of PCR protocols , it is ironic 8.4 Chapter 8 : In Vitro Amplification of DNA by PCR.
Page 8-5
... DNA simul- taneously . In these cases , an amplification reaction termed " multiplex PCR " is used that includes ... amplified product can be produced with dNTP concentrations as low as 20 μM -0.5-1.0 pmole of an amplified fragment ~ 1 ...
... DNA simul- taneously . In these cases , an amplification reaction termed " multiplex PCR " is used that includes ... amplified product can be produced with dNTP concentrations as low as 20 μM -0.5-1.0 pmole of an amplified fragment ~ 1 ...
Page 8-6
... amplified slightly less efficiently than linear DNAs . Although the size of the template DNA is not critical , amplification of sequences embedded ... Amplification of DNA by PCR Chapter Preparation and Analysis of Eukaryotic Genomic DNA 6.
... amplified slightly less efficiently than linear DNAs . Although the size of the template DNA is not critical , amplification of sequences embedded ... Amplification of DNA by PCR Chapter Preparation and Analysis of Eukaryotic Genomic DNA 6.
Page 8-8
... DNA polymerases are summarized in Table 8-1 . Details of the isolation and physi- cal properties of the purified ... amplified DNA is very low . If the annealing temper- ature is too low , nonspecific annealing of primers may occur ...
... DNA polymerases are summarized in Table 8-1 . Details of the isolation and physi- cal properties of the purified ... amplified DNA is very low . If the annealing temper- ature is too low , nonspecific annealing of primers may occur ...
Contents
8-6 | |
8-17 | |
8-23 | |
Analysis of Interacting Proteins with Surface Plasmon Resonance Spectroscopy 18 | 8-96 |
INFORMATION PANELS | 8-107 |
INFORMATION PANELS | 8-109 |
Chapter 9 | 8-120 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 8-128 |
Exon Trapping and Amplification 11 80 | 11-80 |
INFORMATION PANELS | 11-98 |
Chain Reaction | 11-125 |
Chapter 1 | 12-1 |
Chapter 4 | 12-4 |
Chapter 12 | 12-12 |
Chapter 16 | 12-16 |
INTRODUCTION | 12-32 |
Chapter 15 | 9-15 |
Chapter 18 | 9-18 |
INTRODUCTION | 9-25 |
PROTOCOLS | 9-33 |
INFORMATION PANELS | 9-76 |
1 | 10-1 |
Chapter 10 | 10-10 |
Volume 1 | 10-11 |
Volume 2 | 10-16 |
INFORMATION PANELS | 10-43 |
Chapter 3 | 11-3 |
Chapter 5 | 11-5 |
Chapter 11 | 11-7 |
Chapter 8 | 11-8 |
PROTOCOLS | 11-13 |
INFORMATION PANELS | 12-94 |
1 | 12-115 |
Chapter 13 | 13-13 |
INFORMATION PANELS | 13-63 |
Purification of Closed Circular DNA by Equilibrium Centrifugation in CsClEthidium 1 69 | 13-69 |
INFORMATION PANELS | 14-1 |
Chapter 2 | 14-2 |
Chapter 14 | 14-3 |
INFORMATION PANELS | 14-9 |
INFORMATION PANELS | 7 |
1 | 1-1 |
Chapter 7 | 1-7 |
Eukaryotic Cells | 1-17 |
Other editions - View all
Molecular Cloning: Selected Applications in Medicine and Biology Gregory G. Brown Limited preview - 2011 |
Common terms and phrases
Acad agarose gel aliquots amplified DNA annealing Appendix bacteriophage M13 bands base Biol BioTechniques catalyzed cDNA clones cDNA libraries cells centrifuge Chapter cleavage concentration containing cycle ddNTP denatured detection digestion DNA fragments DNA sequencing DNA templates dNTPs double-stranded DNA efficiency Escherichia coli ethanol exonuclease gel electrophoresis gene H₂O hybridization information panel Klenow fragment labeled ligation method microfuge tube microtiter plate minutes molecules mRNA mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products phagemid plaques plasmid plasmid DNA pmoles polyacrylamide gel polymerase chain reaction polymerization polynucleotide kinase preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase room temperature samples screening Sequenase sequencing gel sequencing reactions single-stranded DNA Step strand synthesis target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro wild-type µg/ml
Popular passages
Page 9-91 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 14-37 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-88 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-92 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-87 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 8-120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 8-120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-87 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-103 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-88 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.