Molecular cloning: a laboratory manual, Volume 2 |
From inside the book
Results 1-5 of 13
Page 8-1
... Cloning 8.25 3 Removal of Oligonucleotides and Excess dNTPs from Amplified DNA by Ultrafiltration Introduction to Cloning PCR Products ( Protocols 4-7 ) 8.27 8.30 4 Blunt - end Cloning of PCR Products 8.32 5 Cloning PCR Products into T ...
... Cloning 8.25 3 Removal of Oligonucleotides and Excess dNTPs from Amplified DNA by Ultrafiltration Introduction to Cloning PCR Products ( Protocols 4-7 ) 8.27 8.30 4 Blunt - end Cloning of PCR Products 8.32 5 Cloning PCR Products into T ...
Page 8-30
... Cloning PCR Products CLONING AM AMPLIFIED SEGMENTS OF DNA GENERATED BY PCRS into plasmid or phagemid vectors turns out to be easier said than done . Many products of PCR are recalcitrant to cloning by the usual methods because ...
... Cloning PCR Products CLONING AM AMPLIFIED SEGMENTS OF DNA GENERATED BY PCRS into plasmid or phagemid vectors turns out to be easier said than done . Many products of PCR are recalcitrant to cloning by the usual methods because ...
Page 8-31
... Cloning into T vectors is reportedly 50 times more efficient than blunt - end cloning of amplified DNA fragments ( Holton and Graham 1991 ; Marchuk et al . 1991 ) . This is almost equal to the efficiency of cloning that can be achieved ...
... Cloning into T vectors is reportedly 50 times more efficient than blunt - end cloning of amplified DNA fragments ( Holton and Graham 1991 ; Marchuk et al . 1991 ) . This is almost equal to the efficiency of cloning that can be achieved ...
Page 8-35
... cloning kits ( e.g. , pCR - Script [ SK + ] from Stratagene ; pCRII in the TA Cloning kit from Invitrogen ; pGEM - T from Promega ) ( Hengen 1995a ) . MATERIALS Enzymes and Buffers Gels Bacteriophage T4 DNA ligase Agarose gel Please see ...
... cloning kits ( e.g. , pCR - Script [ SK + ] from Stratagene ; pCRII in the TA Cloning kit from Invitrogen ; pGEM - T from Promega ) ( Hengen 1995a ) . MATERIALS Enzymes and Buffers Gels Bacteriophage T4 DNA ligase Agarose gel Please see ...
Page 8-36
... cloned fragments . 6. Confirm the identity of the cloned fragments by DNA sequencing , restriction mapping , or Southern hybridization . Protocol 6 Cloning PCR Products by Addition of Restriction Sites 8.36 Chapter 8 : In Vitro ...
... cloned fragments . 6. Confirm the identity of the cloned fragments by DNA sequencing , restriction mapping , or Southern hybridization . Protocol 6 Cloning PCR Products by Addition of Restriction Sites 8.36 Chapter 8 : In Vitro ...
Contents
8-6 | |
8-17 | |
8-23 | |
Analysis of Interacting Proteins with Surface Plasmon Resonance Spectroscopy 18 | 8-96 |
INFORMATION PANELS | 8-107 |
INFORMATION PANELS | 8-109 |
Chapter 9 | 8-120 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 8-128 |
Exon Trapping and Amplification 11 80 | 11-80 |
INFORMATION PANELS | 11-98 |
Chain Reaction | 11-125 |
Chapter 1 | 12-1 |
Chapter 4 | 12-4 |
Chapter 12 | 12-12 |
Chapter 16 | 12-16 |
INTRODUCTION | 12-32 |
Chapter 15 | 9-15 |
Chapter 18 | 9-18 |
INTRODUCTION | 9-25 |
PROTOCOLS | 9-33 |
INFORMATION PANELS | 9-76 |
1 | 10-1 |
Chapter 10 | 10-10 |
Volume 1 | 10-11 |
Volume 2 | 10-16 |
INFORMATION PANELS | 10-43 |
Chapter 3 | 11-3 |
Chapter 5 | 11-5 |
Chapter 11 | 11-7 |
Chapter 8 | 11-8 |
PROTOCOLS | 11-13 |
INFORMATION PANELS | 12-94 |
1 | 12-115 |
Chapter 13 | 13-13 |
INFORMATION PANELS | 13-63 |
Purification of Closed Circular DNA by Equilibrium Centrifugation in CsClEthidium 1 69 | 13-69 |
INFORMATION PANELS | 14-1 |
Chapter 2 | 14-2 |
Chapter 14 | 14-3 |
INFORMATION PANELS | 14-9 |
INFORMATION PANELS | 7 |
1 | 1-1 |
Chapter 7 | 1-7 |
Eukaryotic Cells | 1-17 |
Other editions - View all
Molecular Cloning: Selected Applications in Medicine and Biology Gregory G. Brown Limited preview - 2011 |
Common terms and phrases
Acad agarose gel aliquots amplified DNA annealing Appendix bacteriophage M13 bands base Biol BioTechniques catalyzed cDNA clones cDNA libraries cells centrifuge Chapter cleavage concentration containing cycle ddNTP denatured detection digestion DNA fragments DNA sequencing DNA templates dNTPs double-stranded DNA efficiency Escherichia coli ethanol exonuclease gel electrophoresis gene H₂O hybridization information panel Klenow fragment labeled ligation method microfuge tube microtiter plate minutes molecules mRNA mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products phagemid plaques plasmid plasmid DNA pmoles polyacrylamide gel polymerase chain reaction polymerization polynucleotide kinase preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase room temperature samples screening Sequenase sequencing gel sequencing reactions single-stranded DNA Step strand synthesis target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro wild-type µg/ml
Popular passages
Page 9-91 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 14-37 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-88 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-92 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-87 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 8-120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 8-120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-87 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-103 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-88 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.