Molecular cloning: a laboratory manual, Volume 2 |
From inside the book
Results 1-5 of 10
Page 8-6
... genomic DNA , up to 1.0 μg of DNA is utilized per reaction , an amount that contains ~ 3 x 105 copies of a single - copy autosomal gene . The typi- cal amounts of yeast , bacterial , and plasmid DNAs used per reaction are 10 ng , 1 ng ...
... genomic DNA , up to 1.0 μg of DNA is utilized per reaction , an amount that contains ~ 3 x 105 copies of a single - copy autosomal gene . The typi- cal amounts of yeast , bacterial , and plasmid DNAs used per reaction are 10 ng , 1 ng ...
Page 8-13
... genomes is random . This is not the case because of bias in codon usage ( Lathe 1985 ) and because a significant fraction of the genome is composed of repetitive DNA sequences and gene families ( Bains 1994 ) . To minimize problems of ...
... genomes is random . This is not the case because of bias in codon usage ( Lathe 1985 ) and because a significant fraction of the genome is composed of repetitive DNA sequences and gene families ( Bains 1994 ) . To minimize problems of ...
Page 8-14
... genomic DNA to be easily distinguished . When a short sequence of amino acids has been obtained by sequencing a purified protein , pools of degenerate oligonucleotides containing all possible coding combinations can be used to amplify ...
... genomic DNA to be easily distinguished . When a short sequence of amino acids has been obtained by sequencing a purified protein , pools of degenerate oligonucleotides containing all possible coding combinations can be used to amplify ...
Page 8-18
... genomic templates is often more efficient if the oligonucleotide primers are purified by chromatography on commercially available resins ( e.g. , NENSORB , NEN Life Science Products ) or by denaturing polyacrylamide gel electrophoresis ...
... genomic templates is often more efficient if the oligonucleotide primers are purified by chromatography on commercially available resins ( e.g. , NENSORB , NEN Life Science Products ) or by denaturing polyacrylamide gel electrophoresis ...
Page 8-20
... genomic DNA , 100 μg / ml ; yeast genomic DNA , 1 μg / ml ; bacterial genomic DNA , 0.1 μg / ml ; and plasmid DNA , 1-5 ng / ml . Barrier tips for automatic micropipetting device Microfuge tubes ( 0.5 ml , thin - walled for ...
... genomic DNA , 100 μg / ml ; yeast genomic DNA , 1 μg / ml ; bacterial genomic DNA , 0.1 μg / ml ; and plasmid DNA , 1-5 ng / ml . Barrier tips for automatic micropipetting device Microfuge tubes ( 0.5 ml , thin - walled for ...
Contents
8-6 | |
8-17 | |
8-23 | |
Analysis of Interacting Proteins with Surface Plasmon Resonance Spectroscopy 18 | 8-96 |
INFORMATION PANELS | 8-107 |
INFORMATION PANELS | 8-109 |
Chapter 9 | 8-120 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 8-128 |
Exon Trapping and Amplification 11 80 | 11-80 |
INFORMATION PANELS | 11-98 |
Chain Reaction | 11-125 |
Chapter 1 | 12-1 |
Chapter 4 | 12-4 |
Chapter 12 | 12-12 |
Chapter 16 | 12-16 |
INTRODUCTION | 12-32 |
Chapter 15 | 9-15 |
Chapter 18 | 9-18 |
INTRODUCTION | 9-25 |
PROTOCOLS | 9-33 |
INFORMATION PANELS | 9-76 |
1 | 10-1 |
Chapter 10 | 10-10 |
Volume 1 | 10-11 |
Volume 2 | 10-16 |
INFORMATION PANELS | 10-43 |
Chapter 3 | 11-3 |
Chapter 5 | 11-5 |
Chapter 11 | 11-7 |
Chapter 8 | 11-8 |
PROTOCOLS | 11-13 |
INFORMATION PANELS | 12-94 |
1 | 12-115 |
Chapter 13 | 13-13 |
INFORMATION PANELS | 13-63 |
Purification of Closed Circular DNA by Equilibrium Centrifugation in CsClEthidium 1 69 | 13-69 |
INFORMATION PANELS | 14-1 |
Chapter 2 | 14-2 |
Chapter 14 | 14-3 |
INFORMATION PANELS | 14-9 |
INFORMATION PANELS | 7 |
1 | 1-1 |
Chapter 7 | 1-7 |
Eukaryotic Cells | 1-17 |
Other editions - View all
Molecular Cloning: Selected Applications in Medicine and Biology Gregory G. Brown Limited preview - 2011 |
Common terms and phrases
Acad agarose gel aliquots amplified DNA annealing Appendix bacteriophage M13 bands base Biol BioTechniques catalyzed cDNA clones cDNA libraries cells centrifuge Chapter cleavage concentration containing cycle ddNTP denatured detection digestion DNA fragments DNA sequencing DNA templates dNTPs double-stranded DNA efficiency Escherichia coli ethanol exonuclease gel electrophoresis gene H₂O hybridization information panel Klenow fragment labeled ligation method microfuge tube microtiter plate minutes molecules mRNA mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products phagemid plaques plasmid plasmid DNA pmoles polyacrylamide gel polymerase chain reaction polymerization polynucleotide kinase preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase room temperature samples screening Sequenase sequencing gel sequencing reactions single-stranded DNA Step strand synthesis target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro wild-type µg/ml
Popular passages
Page 9-91 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 14-37 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-88 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-92 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-87 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 8-120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 8-120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-87 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-103 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-88 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.