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The Enhancement of Contrast
The Preservation of Specimens
Examples of the Application of Electron Microscopy to the Study
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absorbance absorption acid appears applied atoms axis binding birefringence boundary buffer calculated cell charge Chem chromophores complex concentration constant containing contrast corrected corresponding curve dependence determined dielectric difference diffusion dipole direction discussed distribution effects electric electric field electron electrophoresis elution emission energy equation equilibrium example excitation experimental experiments factor flow fluorescence fraction frequency function gel filtration given groups important increase indicates intensity interactions ionic ions length light limited macromolecules measured method migration mobility molecular molecules observed obtained occurs optical particles patterns peaks perturbation phase phenolic plot polarization position possible preparation present produced protein range ratio reaction reference relative relaxation respectively rotation sample separation serum albumin shift shown single solution solvent specimen spectra spectrum strength structure studies technique temperature theory tion tryptophan unit usually values volume wavelength weight yield zone