Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 200
... curve is divided into the apparent spectrum to yield the corrected curve ( e.g. , Fig . 7 ) . 80 70 60 60 Relative intensity or E ( X ) 50 50 40 30 20 10 O 200 300 400 500 Wavelength ( mμ ) 600 FIG . 9. Light source calibration curve ...
... curve is divided into the apparent spectrum to yield the corrected curve ( e.g. , Fig . 7 ) . 80 70 60 60 Relative intensity or E ( X ) 50 50 40 30 20 10 O 200 300 400 500 Wavelength ( mμ ) 600 FIG . 9. Light source calibration curve ...
Page 201
... curve of the lamp ( in photons emitted per wavelength interval ) . A plot of S ( A ) vs. λ is given by dividing the recorded curve by the known curve for the lamp . 5.0- ( Y ) S 4.0 3.0 2.0 1.0 ΟΙ 300 400 500 Wavelength ( mμ ) 600 FIG ...
... curve of the lamp ( in photons emitted per wavelength interval ) . A plot of S ( A ) vs. λ is given by dividing the recorded curve by the known curve for the lamp . 5.0- ( Y ) S 4.0 3.0 2.0 1.0 ΟΙ 300 400 500 Wavelength ( mμ ) 600 FIG ...
Page 381
... curve corre- sponding to the ideal boundary has the form of a Gaussian distribution of the type expected from simple diffusion , the presence of the electric field transporting the whole curve along the electrophoresis column at a rate ...
... curve corre- sponding to the ideal boundary has the form of a Gaussian distribution of the type expected from simple diffusion , the presence of the electric field transporting the whole curve along the electrophoresis column at a rate ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone