Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 73
... density at a single point in the unit cell . By systematically varying say x and y while keeping z constant , the electron density may be mapped over a plane section through the unit cell . If the process is repeated for a series of z ...
... density at a single point in the unit cell . By systematically varying say x and y while keeping z constant , the electron density may be mapped over a plane section through the unit cell . If the process is repeated for a series of z ...
Page 118
... density of a sample solution of total chromophore concen- tration c , measured in a 1 - cm cell , is given by the sum of the optical densities of the ionized and un - ionized forms . In addition to being a function of chromophore ...
... density of a sample solution of total chromophore concen- tration c , measured in a 1 - cm cell , is given by the sum of the optical densities of the ionized and un - ionized forms . In addition to being a function of chromophore ...
Page 191
... density is given by OD = log 100/95 = 0.022 . Under these conditions the optical density per centimeter path length of solution , measured in a 1 X 1 cm fluorescence cell , is 0.022 / 0.5 = 0.044 . However , if an 0.29 X 0.29 cm ...
... density is given by OD = log 100/95 = 0.022 . Under these conditions the optical density per centimeter path length of solution , measured in a 1 X 1 cm fluorescence cell , is 0.022 / 0.5 = 0.044 . However , if an 0.29 X 0.29 cm ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone