Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 13
... discussed below in Section III , B . Lithium , beryllium , and boron are elements of lower atomic number than carbon , from which films of lower electron - scattering power could , at least in principle , be prepared . To date , no ...
... discussed below in Section III , B . Lithium , beryllium , and boron are elements of lower atomic number than carbon , from which films of lower electron - scattering power could , at least in principle , be prepared . To date , no ...
Page 247
... discussed . They can yield accurate and reliable information about the molecular architecture of proteins . However , pro- tein structure must always be considered in connection with biological function . This is especially important ...
... discussed . They can yield accurate and reliable information about the molecular architecture of proteins . However , pro- tein structure must always be considered in connection with biological function . This is especially important ...
Page 481
... discussed in Chapter 8 . Quantitative interpretation of gel filtration data on rapidly reacting systems is based largely on the theoretical discussions of the forms of boundaries in freely migrating systems devoid of diffusion . Since ...
... discussed in Chapter 8 . Quantitative interpretation of gel filtration data on rapidly reacting systems is based largely on the theoretical discussions of the forms of boundaries in freely migrating systems devoid of diffusion . Since ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone