Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 255
... Relaxation Effects Many protein reactions consist of a number of elementary steps . Since most of these are accompanied by an appreciable enthalpy change ( an exception being the ionization of carboxyl groups ) , a spectrum of re ...
... Relaxation Effects Many protein reactions consist of a number of elementary steps . Since most of these are accompanied by an appreciable enthalpy change ( an exception being the ionization of carboxyl groups ) , a spectrum of re ...
Page 304
... RELAXATION TIMES In the discussion presented hitherto , it is tacitly assumed that the dielectric relaxation is characterized by only one relaxation time . The theory described above is therefore referred to as the Debye theory ...
... RELAXATION TIMES In the discussion presented hitherto , it is tacitly assumed that the dielectric relaxation is characterized by only one relaxation time . The theory described above is therefore referred to as the Debye theory ...
Page 320
... relaxation in the neighborhood of 20 kc and would not contribute any dielectric increment in the 1 mc region . Takashima and Schwan ( 1959 ) investigated the dielectric constant of ovalbumin between 100 cps and 200 kc but no additional ...
... relaxation in the neighborhood of 20 kc and would not contribute any dielectric increment in the 1 mc region . Takashima and Schwan ( 1959 ) investigated the dielectric constant of ovalbumin between 100 cps and 200 kc but no additional ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone