Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 159
... sample absorption , instrument sensitivity , or absorption by air . There are several reasons for determining a ... sample . The sample is measured against the reference before the pH of the sample has been altered ( this is the refer ...
... sample absorption , instrument sensitivity , or absorption by air . There are several reasons for determining a ... sample . The sample is measured against the reference before the pH of the sample has been altered ( this is the refer ...
Page 160
... samples diluted . The pH of the sample solution can often be conveniently altered by adding concentrated acid or base solution to the sample solution using a syringe microburet or a glass rod drawn to a fine tip . Except at ex- tremes ...
... samples diluted . The pH of the sample solution can often be conveniently altered by adding concentrated acid or base solution to the sample solution using a syringe microburet or a glass rod drawn to a fine tip . Except at ex- tremes ...
Page 212
... sample . Both reference source and sample are monitored by photomultiplier tubes , so that two modulated photocurrents are produced . Müller et al . ( 1965 ) have discussed the features desirable in the photomultiplier . The output from ...
... sample . Both reference source and sample are monitored by photomultiplier tubes , so that two modulated photocurrents are produced . Müller et al . ( 1965 ) have discussed the features desirable in the photomultiplier . The output from ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone