Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 44
... shown by the dotted line , while the very irregular outline of the metal cap and shadow is shown by a solid line . Since the deposition of metal may not be radially symmetrical , and since the density of metal after evaporation may not ...
... shown by the dotted line , while the very irregular outline of the metal cap and shadow is shown by a solid line . Since the deposition of metal may not be radially symmetrical , and since the density of metal after evaporation may not ...
Page 192
... shown . In ( b ) , the absorption ( – - ) and emission ( ---- ) spectra of a hypothetical compound are shown together with the triangles representing the transmission of monochromators set at the optimal wavelengths and with slits ...
... shown . In ( b ) , the absorption ( – - ) and emission ( ---- ) spectra of a hypothetical compound are shown together with the triangles representing the transmission of monochromators set at the optimal wavelengths and with slits ...
Page 395
... shown by interacting systems will be discussed in Section III , E . D. ELECTROPHORESIS AS A CRITERION OF PURITY The criteria of purity of simple organic compounds , for example melt- ing point and elementary analysis , are not ...
... shown by interacting systems will be discussed in Section III , E . D. ELECTROPHORESIS AS A CRITERION OF PURITY The criteria of purity of simple organic compounds , for example melt- ing point and elementary analysis , are not ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone