Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 145
... wavelength end of the spectrum of acetyltyrosine ethyl ester is -200 10 € / mμ at 285.0 ± 0.3 mμ . The wavelength shift produced is thus Aλ = —A € / ( de / dλ ) = 120/200 , or 0.6 mμ to the red . Since the maxi- Δλ mum in the difference ...
... wavelength end of the spectrum of acetyltyrosine ethyl ester is -200 10 € / mμ at 285.0 ± 0.3 mμ . The wavelength shift produced is thus Aλ = —A € / ( de / dλ ) = 120/200 , or 0.6 mμ to the red . Since the maxi- Δλ mum in the difference ...
Page 173
... wavelength intensity of fluorescence at a given emission wavelength > optical absorbance at the exciting wavelength area under the corrected emission curve grating correction factor phase shift of modulated light beam frequency of ...
... wavelength intensity of fluorescence at a given emission wavelength > optical absorbance at the exciting wavelength area under the corrected emission curve grating correction factor phase shift of modulated light beam frequency of ...
Page 193
... wavelength absorption band . In such instances , spectrofluorometers will have greater sensitivity . Most of such instruments now available can detect 1 part per billion of quinine sulfate . The sensitivity of fluorescence detection ...
... wavelength absorption band . In such instances , spectrofluorometers will have greater sensitivity . Most of such instruments now available can detect 1 part per billion of quinine sulfate . The sensitivity of fluorescence detection ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone