Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 432
... zone electrophoresis in a density gradient of sucrose or D2O than by moving - boundary electrophoresis . Zone electrophoresis in a den- sity gradient also holds promise as a powerful tool for quantitative characterization of certain ...
... zone electrophoresis in a density gradient of sucrose or D2O than by moving - boundary electrophoresis . Zone electrophoresis in a den- sity gradient also holds promise as a powerful tool for quantitative characterization of certain ...
Page 436
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
Page 438
... zone patterns . While in certain instances the generation of multiple zones due to interaction may be relatively insensitive to protein concentration [ for example , bovine serum albumin in phosphate- borate buffer ( pH 6.1 ) ] , in ...
... zone patterns . While in certain instances the generation of multiple zones due to interaction may be relatively insensitive to protein concentration [ for example , bovine serum albumin in phosphate- borate buffer ( pH 6.1 ) ] , in ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone