Viral Vectors: Gene Therapy and Neuroscience ApplicationsMichael G. Kaplitt, Arthur D. Loewy Genetic manipulation of the adult mammalian nervous system is one of the most exciting areas in contemporary neurobiology. The explosive growth of this field has been facilitated by harnessing the power of viruses to transfer genetic material into mammalian cells. Viral Vectors: Gene Therapy and Neuroscience Applications represents the first comprehensive review of viral vector applications to the nervous system by leaders in virology, molecular neurobiology, neuroanatomy, and developmental neurobiology. It serves both as a source of fundamental information for those newly interested in viral vectors and as a compilation of state-of-the-art technologies and applications for more experienced researchers. This work provides expert background information on viral systems, and the broad range of applications will help readers appreciate the current and future impact of viral vectors in both clinical and basic neuroscience. |
Contents
| 1 | |
| 25 | |
| 43 | |
| 55 | |
| 75 | |
Molecular Analysis of Neuronal Physiology Generation of Genetic Models and Gene Therapy of Neurological Disorders | 89 |
Chapter 7 The Use of Defective Herpes Simplex Virus Amplicon Vectors to Modify Neural Cells and the Nervous System | 109 |
Application to the Reconstruction of Neural Circuits in the Injured Mammalian Brain | 119 |
Chapter 14 Virus VectorMediated Transfer of DrugSensitivity Genes for Experimental Brain Tumor Therapy | 239 |
Chapter 15 Brain Tumor Therapy Using Genetically Engineered ReplicationCompetent Virus | 259 |
Chapter 16 Transfer and Expression of Antioncogenes and Paraneoplastic Genes in Normal and Neoplastic Cells in Vitro and in Viva | 275 |
A Review of the Methodology | 293 |
Chapter 18 Pseudorabies Virus Replication and Assembly in the Rodent Central Nervous System | 319 |
A Transneuronal Tracer for Neuroanatomical Studies | 349 |
Chapter 20 Molecular Properties of Alphaherpesviruses Used in Transneuronal Pathway Tracing | 367 |
Chapter 21 Molecular Analysis of Rabies and Pseudorabies Neurotropism | 395 |
Chapter 9 The Use of Herpes Simplex Virus Vectors for Protection from Necrotic Neuron Death | 133 |
Chapter 10 In Viva Promoter Analysis in the Adult Central Nervous System Using Viral Vectors | 157 |
Potential Therapeutic Applications | 173 |
Chapter 12 Transfer and Expression of Potentially Therapeutic Genes into the Mammalian Central Nervous System in Viva Using AdenoAssociated ... | 193 |
Chapter 13 Genetic Modification of Cells with Retrovirus Vectors for Grafting into the Central Nervous System | 211 |
Chapter 22 The Use of Retroviral Vectors in the Study of Cell Lineage and Migration during the Development of the Mammalian Central Nervous Sy... | 411 |
Immortalization of Neural Cells | 435 |
Index | 477 |
Color Plate Section | 487 |
Other editions - View all
Viral Vectors: Gene Therapy and Neuroscience Applications Michael G. Kaplitt,Arthur D. Loewy No preview available - 1995 |
Viral Vectors: Tools for Study and Genetic Manipulation of the Nervous System Michael G. Kaplitt,Arthur D. Loewy No preview available - 1995 |
Viral Vectors: Gene Therapy and Neuroscience Applications Michael G. Kaplitt,Arthur D. Loewy No preview available - 1995 |
Common terms and phrases
Acad activity addition adenovirus amplicon analysis animals approach binding brain Card cell culture cell lines cellular central clones containing culture cytoplasm defective deletion demonstrated differentiation direct disease dopamine early effect efficient encoding envelope enzyme et al expression factor Figure function gene therapy gene transfer genetic genome glucose glycoproteins groups growth helper virus herpes simplex virus host human immortalized increased indicating infection integration labeling levels method mice mouse mRNA mutants Nature nerve nervous system neural neurons Neurosci normal observed occurs packaging potential present primary Proc progenitors promoter protein pseudorabies virus receptor recombinant region replication response retroviral sequences shown signal simplex virus type specific spread strain structure studies suggest terminal tissue transcription transneuronal transplantation transport treatment tumor Ugolini viral vectors virions Virol viruses vitro vivo wild-type
Popular passages
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