Proteomics: From Protein Sequence to Function, Page 967; Page 2001Following the announcement of the draft sequence of the human genome and the completion of many others, attention is now increasingly turning to the analysis of the proteins encoded by genomes - proteomics. This is a rapidly emerging research area and this book reviews recent developments in proteomics and compares them with current genomics technologies. The book is written by experts, from academia and industry, who review their area in a concise yet easy to understand style, providing a comprehensive review volume that integrates genomics with proteomics. This is the first book to address the subject at an undergraduate level. |
Contents
The characterization of the protein complement of a specific cell type | 13 |
Summary | 19 |
Twodimensional polyacrylamide gel eletrophoresis for proteome analysis | 43 |
Detecting proteins in polyacrylamide gels and on electroblot membranes | 65 |
Eickhoff Holger MaxPlanckInstitute of Molecular Genetics Ihnestrasse | 73 |
Mass spectrometrybased methods for protein identification | 87 |
Evers Stefan F HoffmannLa Roche Ltd PRPID Building 6916 Basel | 99 |
Image analysis of twodimensional gels 131 333 | 131 |
technical and informatic solutions for high | 171 |
a complete approach to automating proteome | 193 |
Novel approaches to protein expression analysis | 207 |
Application of proteome analysis to drug development and toxicology | 225 |
Phage antibodies as tools for proteomics | 237 |
Glycobiology and proteomics | 257 |
Proteomics in an academic environment | 277 |
Proteomics as tool for plant genetics and breeding | 289 |
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Common terms and phrases
2-DE gels Aebersold amino acid Anal analysis of protein analyzed antibodies antigen application approach automated Biochem Biol biological blotting cDNA library cell characterization Chem clones colloidal colloidal gold complex Coomassie blue detection differential digestion electroblotted Electrophoresis 19 electrospray electrospray ionization expression profiling Figure fluorescent fragment ion function gel images gene expression genetic genome genome sequencing glycan glycoproteins glycosylation Görg high-throughput Hochstrasser human hybridization immobilized ionization IPG strips Klose labeling MALDI MALDI-MS Mann mapping mass spectrometry matching membranes methods microarrays mixtures modifications molecular MPIs mRNA MS/MS patterns Patton peptide mass peptide mass fingerprinting pH gradients phage antibodies phosphopeptides phosphorylation polyacrylamide gels potential protein expression protein identification protein spots proteins separated proteome analysis proteomics PVDF quantitative Rabilloud reagents robot sample scanning sensitivity sequence databases sequence tags silver staining specific spectra spectrum SYPRO target techniques throughput tissue two-dimensional electrophoresis two-dimensional gel electrophoresis Yates
Popular passages
Page 305 - Klose, J. (1975) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals.