Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 16
Page 16-76
... Biol . 7 : 1568 . Kaufman , R.J. and P.A. Sharp . 1982a . Amplification and expression of se- quences cotransfected with a modular dihydrofolate reductase complemen- tary DNA gene . J. Mol . Biol . 159 : 601 . - 1982b . Construction of ...
... Biol . 7 : 1568 . Kaufman , R.J. and P.A. Sharp . 1982a . Amplification and expression of se- quences cotransfected with a modular dihydrofolate reductase complemen- tary DNA gene . J. Mol . Biol . 159 : 601 . - 1982b . Construction of ...
Page 16-78
... Biol . 2 : 161 . 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells . Mol . Cell . Biol . 3 : 280 . 1985. Bacteriophage lambda vec- tor for transducing a cDNA clone li- brary into mammalian cells ...
... Biol . 2 : 161 . 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells . Mol . Cell . Biol . 3 : 280 . 1985. Bacteriophage lambda vec- tor for transducing a cDNA clone li- brary into mammalian cells ...
Page 16-80
... Biol . 6 : 1074 . Simonsen , C.C. and A.D. Levinson . 1983 . Isolation and expression of an altered mouse dihydrofolate reductase cDNA . Proc . Natl . Acad . Sci . 80 : 2495 . Simonsen , C.C. , H.M. Shepard , P.W. Gray , D.W. Leung , D ...
... Biol . 6 : 1074 . Simonsen , C.C. and A.D. Levinson . 1983 . Isolation and expression of an altered mouse dihydrofolate reductase cDNA . Proc . Natl . Acad . Sci . 80 : 2495 . Simonsen , C.C. , H.M. Shepard , P.W. Gray , D.W. Leung , D ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml