Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-12
... pH . Protein A is remarkably resilient and withstands repeated cycles of exposure to low pH extremely well ; it can ... Cl ( pH 8.0 ) according to the manufacturer's instructions . Each milliliter of swollen gel can adsorb between 10 mg ...
... pH . Protein A is remarkably resilient and withstands repeated cycles of exposure to low pH extremely well ; it can ... Cl ( pH 8.0 ) according to the manufacturer's instructions . Each milliliter of swollen gel can adsorb between 10 mg ...
Page 18-45
... Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see page 18.38 ) : The first two washes are carried out with RIPA buffer . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . 5. Incubate the resuspended beads ...
... Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see page 18.38 ) : The first two washes are carried out with RIPA buffer . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . 5. Incubate the resuspended beads ...
Page B-26
... ( pH 7.5 ) 20 mM magnesium acetate 100 μg / ml bovine serum albumin ( Fraction V ; Sigma ) 1 mм ẞ - mercaptoethanol This buffer is used with restriction enzymes . 10 × Klenow buffer 0.5 M Tris Cl ( pH 7.6 ) 0.1 M MgCl2 The Klenow fragment ...
... ( pH 7.5 ) 20 mM magnesium acetate 100 μg / ml bovine serum albumin ( Fraction V ; Sigma ) 1 mм ẞ - mercaptoethanol This buffer is used with restriction enzymes . 10 × Klenow buffer 0.5 M Tris Cl ( pH 7.6 ) 0.1 M MgCl2 The Klenow fragment ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml