Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 41
Page B-23
... EDTA 0.5X : 0.045 м Tris - borate 0.001 M EDTA Alkalineb Tris - glycine 1x : 50 mn NaOH 1 mM EDTA 1x : 25 mM Tris 250 mM glycine 0.1 % SDS Concentrated stock solution ( per liter ) 50 × : 242 g Tris base 57.1 ml glacial acetic acid 100 ...
... EDTA 0.5X : 0.045 м Tris - borate 0.001 M EDTA Alkalineb Tris - glycine 1x : 50 mn NaOH 1 mM EDTA 1x : 25 mM Tris 250 mM glycine 0.1 % SDS Concentrated stock solution ( per liter ) 50 × : 242 g Tris base 57.1 ml glacial acetic acid 100 ...
Page E-39
... EDTA ( pH 8.0 ) . 3. Rinse the tubing thoroughly in distilled water . 4. Boil for 10 minutes in 1 mм EDTA ( pH 8.0 ) . 5. Allow the tubing to cool , and then store it at 4 ° C . Be sure that the tubing is always submerged . From this ...
... EDTA ( pH 8.0 ) . 3. Rinse the tubing thoroughly in distilled water . 4. Boil for 10 minutes in 1 mм EDTA ( pH 8.0 ) . 5. Allow the tubing to cool , and then store it at 4 ° C . Be sure that the tubing is always submerged . From this ...
Page 1-44
... EDTA ) , preparation of buffer , B.20 TEACI . See Tetraethylammonium chloride TEMED ( N , N , N ' , N ' - tetramethylethyl- enediamine ) , use in polyacryl- amide gel electrophoresis , 6.36 , 18.49 TEN buffer for ion - exchange method ...
... EDTA ) , preparation of buffer , B.20 TEACI . See Tetraethylammonium chloride TEMED ( N , N , N ' , N ' - tetramethylethyl- enediamine ) , use in polyacryl- amide gel electrophoresis , 6.36 , 18.49 TEN buffer for ion - exchange method ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml