Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-27
... EcoRI EBNA- ( 4700 bp ) 1000 ori HSV EcoRI 6000 pro- moter Ptk p205 ( 8.1 kb ) Nrul 2000 EcoRI hyg 5000 AAAA EBV oriP 3000 HSV polyA Nari 4000 FIGURE 16.6B p205 carries the Epstein - Barr virus origin P ( EBV oriP ) and expresses a ...
... EcoRI EBNA- ( 4700 bp ) 1000 ori HSV EcoRI 6000 pro- moter Ptk p205 ( 8.1 kb ) Nrul 2000 EcoRI hyg 5000 AAAA EBV oriP 3000 HSV polyA Nari 4000 FIGURE 16.6B p205 carries the Epstein - Barr virus origin P ( EBV oriP ) and expresses a ...
Page 16-11
... EcoRI 0 Hindill 29 I BamHI 375 Pvull 950 1000 HSV EcoRI 1055 Bgill 1200 pTK2 ( 7.9 kb ) Ptk AAAA HSV tk Sacl 1690 2000 HSV polyA - Pvull 2975 HSV 3000 EcoRI 3455 BamHI 3975 4000 FIGURE 16.1A PTK2 is a derivative of pBR322 that carries a ...
... EcoRI 0 Hindill 29 I BamHI 375 Pvull 950 1000 HSV EcoRI 1055 Bgill 1200 pTK2 ( 7.9 kb ) Ptk AAAA HSV tk Sacl 1690 2000 HSV polyA - Pvull 2975 HSV 3000 EcoRI 3455 BamHI 3975 4000 FIGURE 16.1A PTK2 is a derivative of pBR322 that carries a ...
Page 16-27
... EcoRI 1000 EBNA - 1 ( A700 bp ) ori HSV EcoRI Ptk pro- 6000 moter p205 ( 8.1 kb ) Nrul 2000 EcoRI hyg AAAA EBV oriP 3000 5000 HSV polyA Narl 4000 FIGURE 16.6B p205 carries the Epstein - Barr virus origin P ( EBV oriP ) and expresses a ...
... EcoRI 1000 EBNA - 1 ( A700 bp ) ori HSV EcoRI Ptk pro- 6000 moter p205 ( 8.1 kb ) Nrul 2000 EcoRI hyg AAAA EBV oriP 3000 5000 HSV polyA Narl 4000 FIGURE 16.6B p205 carries the Epstein - Barr virus origin P ( EBV oriP ) and expresses a ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml