Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 15
Page 18-36
... MgCl2 2 mм dithiothreitol 50 mm potassium phosphate ( pH 7.8 ) 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract , the eyes , and skin . PMSF may ...
... MgCl2 2 mм dithiothreitol 50 mm potassium phosphate ( pH 7.8 ) 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract , the eyes , and skin . PMSF may ...
Page B-26
... MgCl2 The Klenow fragment of E. coli DNA polymerase I also works in a wide variety of other buffers such as restriction enzyme buffers , bacteriophage T4 DNA polymerase buffer , etc. ( see Chapter 5 ) . 10x Nick - translation buffer ...
... MgCl2 The Klenow fragment of E. coli DNA polymerase I also works in a wide variety of other buffers such as restriction enzyme buffers , bacteriophage T4 DNA polymerase buffer , etc. ( see Chapter 5 ) . 10x Nick - translation buffer ...
Page F-8
... MgCl2 100 mм dithiothreitol 2 mg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) This buffer should be stored in small aliquots at -20 ° C . 2. Add 2 units of bacteriophage T4 polynucleotide kinase and incubate the ...
... MgCl2 100 mм dithiothreitol 2 mg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) This buffer should be stored in small aliquots at -20 ° C . 2. Add 2 units of bacteriophage T4 polynucleotide kinase and incubate the ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml