Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-32
... Place 1 ml each of the induced and uninduced culture in separate microfuge tubes . Centrifuge at 12,000g for 1 minute at 4 ° C in a microfuge to pellet the cells . Discard the supernatant . b . Suspend the cell pellets in 100 μl of a ...
... Place 1 ml each of the induced and uninduced culture in separate microfuge tubes . Centrifuge at 12,000g for 1 minute at 4 ° C in a microfuge to pellet the cells . Discard the supernatant . b . Suspend the cell pellets in 100 μl of a ...
Page 18-66
... Place the final three sheets of 3MM paper on the gel , again making sure that they are exactly aligned and that no air bubbles are trapped . 6. Place the upper electrode ( which will become the cathode ) on top of the stack , graphite ...
... Place the final three sheets of 3MM paper on the gel , again making sure that they are exactly aligned and that no air bubbles are trapped . 6. Place the upper electrode ( which will become the cathode ) on top of the stack , graphite ...
Page E-26
... place . 3. In a darkroom , place the sample in a light - tight X - ray film holder and cover it with a sheet of X - ray film . If preflashed film is used , the preexposed side should face the sample , unless an intensifying screen is ...
... place . 3. In a darkroom , place the sample in a light - tight X - ray film holder and cover it with a sheet of X - ray film . If preflashed film is used , the preexposed side should face the sample , unless an intensifying screen is ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml