Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-79
... acetate in the translation reactions varies from preparation to preparation of reticulocyte lysate and from mRNA to mRNA . The amount of magnesium acetate required to achieve the maximum efficiency of transla- tion should be determined ...
... acetate in the translation reactions varies from preparation to preparation of reticulocyte lysate and from mRNA to mRNA . The amount of magnesium acetate required to achieve the maximum efficiency of transla- tion should be determined ...
Page B-13
... acetate ( for alkaline lysis ) 3 M Sodium acetate ( pH 5.2 and pH 7.0 ) 5 M NaCl 10 % Sodium dodecyl sulfate ( SDS ) ( also called sodium lauryl sulfate ) 20 × SSC 20 × SSPE Trichloroacetic acid ( TCA ) 100 % solution 1 M Tris Dissolve ...
... acetate ( for alkaline lysis ) 3 M Sodium acetate ( pH 5.2 and pH 7.0 ) 5 M NaCl 10 % Sodium dodecyl sulfate ( SDS ) ( also called sodium lauryl sulfate ) 20 × SSC 20 × SSPE Trichloroacetic acid ( TCA ) 100 % solution 1 M Tris Dissolve ...
Page B-26
... acetate ( pH 8.0 ) 0.66 м potassium acetate 0.1 м magnesium acetate 5 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 × stock should be divided into small aliquots and stored frozen at -20 ° C . Reverse ...
... acetate ( pH 8.0 ) 0.66 м potassium acetate 0.1 м magnesium acetate 5 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 × stock should be divided into small aliquots and stored frozen at -20 ° C . Reverse ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml