Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-48
... acrylamide ratio increases , reaching a minimum when the ratio is approximately 1:20 . Most SDS - polyacrylamide gels are cast with a molar ratio of bisacrylamide : acrylamide of 1:29 , which has been shown empirically to be capable of ...
... acrylamide ratio increases , reaching a minimum when the ratio is approximately 1:20 . Most SDS - polyacrylamide gels are cast with a molar ratio of bisacrylamide : acrylamide of 1:29 , which has been shown empirically to be capable of ...
Page 18-51
... acrylamide solution of dissolved oxygen ( which retards polymerization ) by degassing . 3. Pour the acrylamide solution into the gap between the glass plates . Leave sufficient space for the stacking gel ( the length of the teeth of the ...
... acrylamide solution of dissolved oxygen ( which retards polymerization ) by degassing . 3. Pour the acrylamide solution into the gap between the glass plates . Leave sufficient space for the stacking gel ( the length of the teeth of the ...
Page B-9
... Acrylamide 40 % Acrylamide Actinomycin D Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with ...
... Acrylamide 40 % Acrylamide Actinomycin D Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml